Journal: Annals of Medicine and Surgery

Article Title: Da-yuan-yin decoction ameliorates inflammatory injury in severe pancreatitis by protecting intestinal mucosal barrier and immune function and preventing intestinal dysbiosis

doi: 10.1097/MS9.0000000000004873

Figure Lengend Snippet: Serum interleukin (IL)-1β and IL-10 expression levels (pg/ml) and cluster of differentiation (CD)3 + , CD4 + , and CD8 + cells from five ileal Peyer’s patches in the sham operation (SO), severe acute pancreatitis (SAP), and da-yuan-yin (DYY) + SAP groups, respectively. Serum IL-1β and IL-10 levels in the SAP and DYY + SAP groups were significantly higher than those in the SO group at 24, 48, and 72 h after surgery (all P < 0.05). Serum IL-1β and IL-10 levels in the DYY + SAP group were significantly lower than those in the SAP group 72 h postoperatively ( t = 4.37, P = 0.003; t = 2.41, P = 0.04, respectively). Levels of CD3 + , CD4 + , and CD8 + cells from ileal Peyer’s patches in the SAP and DYY + SAP groups decreased with time post-surgery. At 72 h postoperatively, the DYY + SAP group had significantly higher levels of CD3 + , CD4 + , and CD8 + cells than the SAP group ( t = 2.58, P = 0.036; t = 2.78, P = 0.027; t = 2.77, P = 0.028, respectively). There were no significant differences in early or late apoptosis in ileal Peyer’s patches 24, 48, and 72 h postoperatively in the SO, SAP, and DYY + SAP groups.

Article Snippet: Single-cell suspension is stained with the following antibodies: Live/Dead Fixable Violet Dead Cell (Cat# 2549272, Invitrogen), anti-Rat CD3 (Cat# G4.18, MultiSciences Biotech Co., Ltd), and anti-Rat CD4 (Cat# OX35, MultiSciences).

Techniques: Expressing

Journal: International Journal of Chronic Obstructive Pulmonary Disease

Article Title: Exploring the Mechanisms of Lijin Fang on Treg/Th17 Cell Imbalance in COPD Based on Network Pharmacology

doi: 10.2147/COPD.S512469

Figure Lengend Snippet: Flow Cytometry Results for Treg (CD3+CD4+FOXP3+) / Th17 (CD3+CD4+IL17) Detection. LJF can reverse the decrease in Treg cells and the upregulation of Th17 cells induced by CSE. ( A ). Percentage of Treg cells in the Control group, CSE group, CSE+LJF-L group, and CSE+LJF-H group. ( B ). Percentage of Th17 cells in the Control group, CSE group, CSE+LJF-L group, and CSE+LJF-H group. (N=3. ***p<0.001, **p<0.01.).

Article Snippet: Following the manufacturer’s instructions, the appropriate volumes of antibodies against CD3 (E-AB-F1228J, Elabscience, China), CD4 (E-AB-F1105C, Elabscience, China), FOXP3 (B369751, BioLegend, China), and IL-17 (17–7177-81, eBioscience, USA) were added and mixed thoroughly.

Techniques: Flow Cytometry, Control

Journal:

Article Title: The Role of the Thymus in Development of Necrotizing Arteritis in Transgenic Rats Carrying the env-pX Gene of Human T-Cell Leukemia Virus Type-I

doi:

Figure Lengend Snippet: Necrotizing arteritis in lethally irradiated WKAH rats reconstituted by env-pX SCs. The affected vessels in these figures are the testicular artery (A–C: original magnifications, ×100; D: immunostaining for CD3, original magnification, ×400) and arterioles in the mediastinum (E and F: original magnifications, ×200). A and E, H&E staining; B, elastica van Gieson staining (disruption of elastic fibers is evident); C and F, phosphotungstic acid hematoxylin staining (fibrinoid necrosis of the arterial wall is evident).

Article Snippet: Specimens with evident vascular lesions were further examined in elastica van Gieson, Masson trichrome, and phosphotungstic acid hematoxylin stainings, and in immunohistochemistry using anti-rat CD3 antibody (Cedarlane).

Techniques: Irradiation, Immunostaining, Staining, Disruption